For studying proliferation and determination of survival of cancer cells after irradiation, the multiple MTT assay,\r\nbased on the reduction of a yellow water soluble tetrazolium salt to a purple water insoluble formazan dye by\r\nliving cells was modified from a single-point towards a proliferation assay. This assay can be performed with a\r\nlarge number of samples in short time using multi-well-plates, assays can be performed semi-automatically with a\r\nmicroplate reader. Survival, the calculated parameter in this assay, is determined mathematically. Exponential\r\ngrowth in both control and irradiated groups was proven as the underlying basis of the applicability of the\r\nmultiple MTT assay. The equivalence to a clonogenic survival assay with its disadvantages such as time\r\nconsumption was proven in two setups including plating of cells before and after irradiation. Three cell lines (A\r\n549, LN 229 and F 98) were included in the experiment to study its principal and general applicability.
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